rabbit polyclonal anti psca antibody  (Santa Cruz Biotechnology)

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: SNPs located in exons.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques:

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: Allele distribution of the 12 selected SNPs.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Control

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: The rs2294008 polymorphism in PSCA . OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques:

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: Genotype distribution of the 8 SNPs.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Control

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA expression pattern in eight cancer and two normal cell lines. OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA expression in OSCC and normal serum.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA protein expression in OSCC and normal tissues.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA expression in OSCC tissues. (A and B) Representative images indicating PSCA expression in tissues from patients with OSCC and PSCA-positive serum (IRS=6; A, 100×; B, 200×). (C and D) PSCA expression in tissues from patients with OSCC and PSCA-negative serum (IRS=2; C, ×100; D, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA expression in normal epithelium. Representative images from normal tissue with an IRS=0 (A, ×100; B, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

doi: 10.3892/ol.2018.8468

Figure Lengend Snippet: PSCA expression in OSCC epithelium with different genotypes. (A and B) Representative images indicating PSCA expression in tissues from OSCC patients presenting the CC genotype (A, ×100; B, ×200). (C and D) Representative images indicating PSCA expression in OSCC patients presenting the CT genotype (C, ×100; D, ×200). Representative images indicating PSCA expression in tissues from OSCC patients presenting the TT genotype (E, ×100; F, ×200). OSCC, oral squamous cell carcinoma; PSCA, prostate stem cell antigen.

Article Snippet: Rabbit polyclonal anti-PSCA antibody was purchased from Abcam (Cambridge, UK).

Techniques: Expressing

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Association between PSCA SNP rs2294008 and risk of cervical cancer.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques:

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Upper panel, representative images were obtained at 400× magnification. The frequency distribution of the CC, CT, and TT genotypes of rs2294008 was 25, 19, and 6, respectively. Bottom, the histogram of the PSCA expression in each genotype. ** P < 0.01.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Identification of Genomic Alterations in Pancreatic Cancer Using Array-Based Comparative Genomic Hybridization

doi: 10.1371/journal.pone.0114616

Figure Lengend Snippet: A. Strong and negative expression of HMGA2. B. Strong and negative expression of PSCA.

Article Snippet: The slides were blocked by 10% normal goat serum for 30 min at 37°C and then incubated with mouse monoclonal antibody against HMGA2 (abcam, Cambridge, MA) and rabbit polyclonal antibody against PSCA (abcam, Cambridge, MA) overnight at 4°C.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Identification of Genomic Alterations in Pancreatic Cancer Using Array-Based Comparative Genomic Hybridization

doi: 10.1371/journal.pone.0114616

Figure Lengend Snippet: Association between PSCA Expression and Clinicopathological Characteristics of the Pancreatic Cancer.

Article Snippet: The slides were blocked by 10% normal goat serum for 30 min at 37°C and then incubated with mouse monoclonal antibody against HMGA2 (abcam, Cambridge, MA) and rabbit polyclonal antibody against PSCA (abcam, Cambridge, MA) overnight at 4°C.

Techniques: Expressing

Journal: Biology of reproduction

Article Title: Identification of distinct populations of prostasomes that differentially express prostate stem cell antigen, annexin A1, and GLIPR2 in humans.

doi: 10.1095/biolreprod.111.095760

Figure Lengend Snippet: FIG. 1. Fractionation by column chromatography. Membrane vesicles were collected from seminal plasma by ultracentrifugation and then separated by Sephacryl S-1000 column chromatography. A) CD9 and PSCA immunoblots of protein-containing fractions. Molecular weight markers are indicated in Mr 3 103. B and C) Representative whole mount transmission electron micrographs of membrane vesicles from pooled void volume fractions (28–34 from A, presented in B) and retained membrane vesicles (pooled fractions 44–54 from A, presented in C). Bars ¼ 500 nm. The data shown are representative of three independent experiments.

Article Snippet: Other blots were probed with mouse anti-human PSCA (clone 7F5; 1:1000; Santa Cruz Biotechnology Inc.), rabbit anti-human PSCA (1:250; Acris Antibodies Inc.), rabbit anti-human GLIPR2 [29] (1:5000), or mouse anti-human annexin A1 (clone 29; 1:250; BD Biosciences) [30].

Techniques: Fractionation, Column Chromatography, Membrane, Clinical Proteomics, Western Blot, Molecular Weight, Transmission Assay

Journal: Biology of reproduction

Article Title: Identification of distinct populations of prostasomes that differentially express prostate stem cell antigen, annexin A1, and GLIPR2 in humans.

doi: 10.1095/biolreprod.111.095760

Figure Lengend Snippet: FIG. 2. Fractionation on sucrose density gradients. Membrane vesicles from the pooled column fractions 44–54 in Figure 1A were loaded either on top (A) or at the bottom (B) of sucrose gradients and centrifuged for 62 h. C) Membrane vesicles were loaded at the bottom of a linear sucrose gradient and centrifuged for 16 h. CD9 and PSCA in gradient fractions were detected by immunoblotting as indicated. Molecular weight markers are indicated (Mr3103) on the right of the image, and the densities of the fractions (g/ml) are shown below the blots. The data shown are representative of six independent experiments.

Article Snippet: Other blots were probed with mouse anti-human PSCA (clone 7F5; 1:1000; Santa Cruz Biotechnology Inc.), rabbit anti-human PSCA (1:250; Acris Antibodies Inc.), rabbit anti-human GLIPR2 [29] (1:5000), or mouse anti-human annexin A1 (clone 29; 1:250; BD Biosciences) [30].

Techniques: Fractionation, Membrane, Western Blot, Molecular Weight

Journal: Biology of reproduction

Article Title: Identification of distinct populations of prostasomes that differentially express prostate stem cell antigen, annexin A1, and GLIPR2 in humans.

doi: 10.1095/biolreprod.111.095760

Figure Lengend Snippet: FIG. 3. Membrane vesicles from high- and low-density fractions are distinct in size but both contain CD9 and PSCA. Two vesicle populations were separated by sucrose gradient fractionation as indicated in Figure 2C. Vesicles from high- and low-density fractions were immobilized, immunogold- labeled for CD9 or PSCA, and analyzed by whole mount transmission electron microscopy. Representative pictures from two independent experiments are shown. Labeled vesicles are indicated by arrows. Bars¼ 500 nm. Vesicle mean sizes and percentages of vesicles labeled for CD9 or PSCA are indicated in Table 1.

Article Snippet: Other blots were probed with mouse anti-human PSCA (clone 7F5; 1:1000; Santa Cruz Biotechnology Inc.), rabbit anti-human PSCA (1:250; Acris Antibodies Inc.), rabbit anti-human GLIPR2 [29] (1:5000), or mouse anti-human annexin A1 (clone 29; 1:250; BD Biosciences) [30].

Techniques: Membrane, Fractionation, Labeling, Transmission Assay, Electron Microscopy

Journal: Biology of reproduction

Article Title: Identification of distinct populations of prostasomes that differentially express prostate stem cell antigen, annexin A1, and GLIPR2 in humans.

doi: 10.1095/biolreprod.111.095760

Figure Lengend Snippet: FIG. 4. PSCA is absent on prostasomes from some individual donors. Prostasomes were collected from the seminal fluid of individual donors (1–12) and immunoblotted for CD9 (A). The samples were probed on parallel blots for PSCA using mouse anti-human PSCA (B) or rabbit anti- human PSCA (C). Molecular weight markers are indicated on the right (Mr 3 103).

Article Snippet: Other blots were probed with mouse anti-human PSCA (clone 7F5; 1:1000; Santa Cruz Biotechnology Inc.), rabbit anti-human PSCA (1:250; Acris Antibodies Inc.), rabbit anti-human GLIPR2 [29] (1:5000), or mouse anti-human annexin A1 (clone 29; 1:250; BD Biosciences) [30].

Techniques: Molecular Weight

Journal: PLoS ONE

Article Title: Positive and Negative Regulation of Prostate Stem Cell Antigen Expression by Yin Yang 1 in Prostate Epithelial Cell Lines

doi: 10.1371/journal.pone.0035570

Figure Lengend Snippet: A. Pten CaP8 and Pten CaP2 cells were incubated in hormone-depleted medium for 48 hours and then treated with 10 nM R1881 or vehicle (upper panels). Pten CaP2 and Pten CaP8 cells were infected with a lentivirus expressing YY1 specific siRNA or dsRed expression virus as control. Cells were then androgen deprived and treated with 10 nM R1881 for 48 hours (lower panels). Cell surface PSCA expression was determined by FACS using a polyclonal rabbit anti PSCA antibody. B. Expression of YY1 in Pten CaP8 and Pten CaP2 cells infected with YY1 siRNA expressing or control lentivirus was determined by Western blot. The blot was re-probed with anti-β-actin as a loading control. C. Quantitative real-time PCR analysis for PSCA expression was conducted on RNA extracted from Pten CaP2 cells in A.

Article Snippet: Cell surface PSCA protein levels were quantified by staining cell lines with rabbit polyclonal anti-PSCA antibody (0.2 μg/ml, Santa Cruz Biotechnology) followed by goat anti-rabbit-APC (1∶1000) and analyzed on a BD Facscalibur instrument using Cellquest Pro® software.

Techniques: Incubation, Infection, Expressing, Virus, Control, Western Blot, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: Positive and Negative Regulation of Prostate Stem Cell Antigen Expression by Yin Yang 1 in Prostate Epithelial Cell Lines

doi: 10.1371/journal.pone.0035570

Figure Lengend Snippet: A. LNCaP cells (2×10 5 /well) were infected in triplicate with YY1siRNA or scrambled control, and evaluated 72 hr later. PSCA message was determined by quantitative real-time PCR analysis. B. The amount of endogenous YY1 protein in YY1siRNA- or scramble control-infected cells was determined by Western blot. Representative analysis of one sample of triplicates is shown. C. PC-3 cells (2×10 5 /well) were infected in triplicate with YY1siRNA or scrambled control, and evaluated 72 hr later. PSCA message was determined by quantitative real-time PCR analysis. One independent repeat of the experiment was conducted with similar results. * = p<0.03. D. Endogenous YY1 protein in YY1siRNA- or scramble control-infected cells was determined by Western blot. Representative analysis of one sample of triplicates is shown.

Article Snippet: Cell surface PSCA protein levels were quantified by staining cell lines with rabbit polyclonal anti-PSCA antibody (0.2 μg/ml, Santa Cruz Biotechnology) followed by goat anti-rabbit-APC (1∶1000) and analyzed on a BD Facscalibur instrument using Cellquest Pro® software.

Techniques: Infection, Control, Real-time Polymerase Chain Reaction, Western Blot